PE-22-28

PE-22-28 is a synthetic truncated analog of Spadin, the active peptide fragment (residues 22–28) derived from the propeptide of human sortilin (a vacuolar protein sorting receptor). Spadin was identified as an endogenous antagonist of TREK-1 (TWIK-related K+ channel 1, encoded by KCNK2), a two-pore domain background potassium channel with high expression in hippocampal and cortical neurons.

TREK-1 is a mechanosensitive, temperature-sensitive, and lipid-regulated channel that normally acts to stabilize neuronal resting membrane potential. Its blockade by PE-22-28 produces rapid (24–48 hour onset) antidepressant-like effects in preclinical models — a pharmacological profile distinct from classical antidepressants that require weeks of administration. Research also indicates TRPV4 modulatory activity, adding to its mechanistic complexity.

• TREK-1 (KCNK2) potassium channel antagonism and neurophysiology
• Rapid-onset antidepressant mechanism research (24–48h)
• Spadin peptide analog pharmacology and structure-activity studies
• TRPV4 modulation and mechanosensitive channel research
• Depression and mood disorder preclinical models
• Comparison with classical antidepressants on onset kinetics

Dose levels referenced by the MVLabs peptide calculator, shown for research reference only:

TREK-1 modulator (spadin analog) studied for fast-acting mood pathways.

Research reference only — not dosing guidance for human use. Use the calculator for other vials or volumes.

Supplied as a lyophilized powder. Store at 2–8 °C on receipt; protect from heat, light and moisture, and avoid excessive agitation. If reconstitution is required for laboratory work, use sterile technique — bacteriostatic water, or 0.6% acetic acid for low-solubility peptides. Protocols vary by research application.